RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
Gibco® KaryoMAX® Colcemid™ Solution in HBSS is a 10 µg/mL N-desacetyl-N-methylocolchicine (Colcemid™) solution made up in Hanks’ Balanced Salt Solution (HBSS). Colcemid™ prevents spindle formation during mitosis, arresting cells in metaphase so that the chromosomes can be separated for cytogenetic studies and in vitro diagnostic procedures. The mechanism of action is similar to that of…
Gibco® Phytohemagglutinin, M form (PHA-M) is a solution made from a crude extract of the red kidney bean Phaseolus vulgaris. This extract is capable of inducing blastogenesis in vitro in various mammalian mononuclear cells when used at a concentration of 1–2% v/v. Each lot of Gibco® Phytohemagglutinin is performance tested at 1.5% using peripheral blood…
Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements.
Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of applications, including reagent preparation, diluting cells for flow cytometry, and as a cell culture reagent. These PBS tablets are not intended for cell culture as they are not tested for sterility. We offer a variety of Gibco® PBS formulations for cell culture…
Gibco® AmnioMAX™ – II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures. Gibco AmnioMAX C-100 Complete Medium is two-part kit for preparation of a complete medium that requires no additional supplementation. Quality and performance testing Every lot of Gibco AmnioMAX…
DMEM (Dulbecco’s Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
Cell-based applications are an integral part of not only basic research, but also biochemical studies, vaccine development, and drug discovery to name a few. Fetal Bovine Serum (FBS) is the most commonly used serum as it provides the most robust culture system for the widest range of cell types. Gibco FBS can provide the cell…
